凌笑梅

北京大学药学院教授，博士生导师，化学生物学系副主任。多年来一直从事仪器分析和药物分析的教学和科研工作，主编了《高等仪器分析实验与技术》，并担任药物分析杂志编委、中国药学会药物分析委员会委员等。...

北京大学药学院教授，博士生导师，化学生物学系副主任。多年来一直从事仪器分析和药物分析的教学和科研工作，主编了《高等仪器分析实验与技术》，并担任药物分析杂志编委、中国药学会药物分析委员会委员等。

北京大学药学院教授，博士生导师，化学生物学系副主任。多年来一直从事仪器分析和药物分析的教学和科研工作，主编了《高等仪器分析实验与技术》，并担任药物分析杂志编委、中国药学会药物分析委员会委员等。对色谱联用技术和高效毛细管电泳在医药领域中的应用做了大量的研究工作，主要研究工作有：（1）药物代谢动力学及代谢组学研究；（2）生物分子之间的相互作用及生物分子与药物分子之间相互作用研究；（3）天然产物及合成化合物中活性成分的定性定量筛选研究；（4）新药质量标准及稳定性研究；（5）手性药物拆分测定；（6）体内、体外脂肪酸含量测定等。发表相关学术论文70余篇。

1、药物分析新技术和新方法研究及其在生物医药领域中的应用研究:采用毛细管电泳方法研究分子间相互作用研究，如生物大分子和小分子、生物大分子与生物大分子相互作用；手性物质的拆分及与生物大分子相互作用研究；毛细管色谱柱固定化技术研究（固定蛋白、细胞等）及在药物研究中的应用。

2、药物代谢动力学及代谢组学研究:采用现代仪器和色谱联用技术，进行药物体内的生物转化、血药浓度-时间曲线、吸收、分布、排泄、与血浆蛋白结合、对药物代谢酶活性影响和药物-药物相互作用及代谢组学研究等。

3、新药质量标准及稳定性研究:创新药物质量标准建立和稳定性研究。

1．Wen-Jing Chen, Pazilaiti.Yakufu,Xiao-Mei Ling, Hui Qi, Jun-Hai Xiao, Ying Wang, N-terminal extracellular tail of CC chemokine receptor 4 (ML40) immobilized on capillary column to study the interactions between ML40 and its potential ligands,Analytical Biochemistry（Revised）

2．Shuyu Zhang, Hui Qi, Pazilaiti. Yakufu, Fang Zhao,Xiaomei Ling, Junhai Xiao, Ying Wang, Screening of thiourea derivatives and carbonyl-2-aminothiazole derivatives for potential CCR4 antagonists using capillary zone electrophoresis,Journal of Chromatography B, 2011, 879: 75-82

3．Wujie Li, Yinling Ma, Zhu Gan,Xiaomei Ling, Zhenjun Yang, Qualitative and quantitative determination of oligonucleotide by non-gel capillary electrophoresis,Chromatographia, 2011, 73: 579-582

4．Lu Pei, Lujia Xie, Qian Lin,Xiaomei Ling, Zhu Guan, Zhenjun Yang, Studies on the adenosine deaminase-catalyzed conversion of adenosine and nucleoside prodrugs by different capillary electrophoresis modes,Analytical Biochemistry, 2011, 414: 131-137

5．Xiaomei Jing, Yihao Bai,Xiaomei Ling, Fangbin Han, Runtao Li, Jingrong Cui, Simultaneous determination of 4-methyl-piperazine-1-carbodithioc acid 3-cyano-3,3-diphenylpropyl ester hydrochloride and its major metabolite in rats by high-performance liquid chromatography,Journal of Chromatography Science, 2010, 48: 125-129

6．Wujie Li, Mou Cao, Lu Pei,Xiaomei Ling, Bingchao Li, Zhenjun Yang, Study on steady-state kinetics of nucleotide analogues incorporation by non-gel capillary electrophoresis,Electrophoresis, 2010, 31: 507-511

7．Wenjing Chen, Wujie Li,Xiaomei Ling, Xiaowei Wang, Junyi Liu, Study on the interaction between HIV reverse transcriptase and its non-nucleoside inhibitor nevirapine by capillary electrophoresis,Journal of Chromatography B, 2010, 878: 1714-1717

8．Siting Wang, Yan Song, Jiao Wang,Xiaomei Ling, Zemei Ge, Runtao Li and Jingrong Cui, Gas-fragmentation study of the novel synthetic zwitterionic drug 3-methyl-9-(2-oxa-2λ5-2H-1,3,2-oxazaphosphorine-2-cyclohexyl)-3,6,9-triazaspiro[5,5]undecane chloride (SLXM-2) by electrospray ionization tandem mass spectrometry,Rapid Communications in Mass Spectrometry, 2010, 24: 1181-1184

9．Jiao Wang, Yan Song, Jianmei Zhang, Xiaona Li,Xiaomei Ling, Runtao Li and Jingrong Cui, LC and LC–MS Study of the Bioavailability and Metabolites of TM208 after Oral and Intravenous Administration to Rat,Chromatographia, 2010, 72: 459-464

10．Shuyu Zhang, Jun Cheng, Wenjing Chen,Xiaomei Ling, Yiying Zhao, Jie Feng , Cheng Xiang , Hong Liang, Interactions between thrombin and natural products of Millettia nitita var. hirsutissima using capillary zone electrophoresis,Journal of Chromatography B, 2009, 877: 4107-4114

11．Detao Kong, Siting Wang,Xiaomei Ling, Runtao Li, Jingrong Cui, Determination of anti-tumor agent TM208, 4-methyl-piperazine-1-carbodithioc acid 3-cyano-3,3-diphenylpropyl ester hydrochloride, in rats by LC,Chromatographia, 2009, 70: 1721-1725

12．Wenjing Chen , Ke Mou, Bo Xu,Xiaomei Ling, Jingrong Cui, Ping Xu, Capillary electrophoresis for screening of 20S proteasome inhibitors,Analytical Biochemistry, 2009, 394(11): 62-67

13．Zhe Sun,Xiaomei Ling, Yingmei Zhang, Linjie Tian, Ying Wang, Interactions of the peptides from secreted human CKLF1 and the N-terminal extracellular tail of CCR4 analyzed by capillary zone electrophoresis,Chromatographia, 2009, 70(1/2): 287-292

14．Wenjing Chen,Xiaomei Ling, Advances of methods of protein immobilization on capillary,Chinese Journal of Analytical Chemistry, 2009, 37(6): 929-934.

15．Yihao Bai, Zhen Chen,Xiaomei Ling, Runtao Li, Jingrong Cui, Determination of chloride 3-methyl-9-(2-oxa-2k5-2H-1,3,2- oxazaphosphorine-cyclohexyl)-3,6,9- triazaspiro [5.5] undecane (SLXM-2) in rats by LC:application to a pharmacokinetic study,Chromatographia, 2009, 69(1-2): 111-115

16．Shuyu Zhang, Ting Yin,Xiaomei Ling, Hong Liang, Yuying Zhao, Interactions between thrombin and natural products of Millettia speciosa Champ. using capillary zone,Electrophoresis,2008, 29(16): 3391-3397

17．Yi Liu, Shuyu Zhang,Xiaomei Ling, Yuzhen Li, Yingmei Zhang, Wenling Han, Ying Wang, Analysis of the interactions between the peptides from secreted human CKLF1 and heparin using capillary zone electrophoresis,Journal of Peptide Science, 2008, 14(8): 984-988

18．Yi Liu, Wujie Li,Xiaomei Ling, Xianyin Lai, Yuzhen Li, Qingying Zhang, Yuying Zhao, Simultaneous determination of the active ingredients in Abelmoschus manihot (L.) Medicus by CZE,Chromatographia,2008, 67(9-10): 819-823

19．Detao Kong,Xiaomei Ling, Fangbin Han, Zemei Ge, and Jingrong Cui, Identification of the metabolite and cytochrome P450 isoforms involved in rat liver microsomal metabolism of TM208,Journal of Chinese Pharmaceutical Sciences, 2008, 17(1): 30-34

20．Zhe Sun,Xiaomei Ling, Wei Sun, Junhai Xiao, Caihua Yin, Ying Wang, Studies on the interactions between lactam analogs and the N-terminal extracellular tail of CC chemokine receptor 4 by CZE (p NA),Electrophoresis,2007, 28(17): 3064-3069

21．Liu Yi,Xiaomei Ling, Fan Hui, Zhong Yingcheng, Li Dan, Wang Ying. Determination of binding constant and binding region of programmed cell death 5-heparin by capillary zone electrophoresis,Journal of Chromatography A, 2007, 1143(1-2): 284-287

22．Xiaomei Jiang,Xiaomei Ling, Fangbin Han, Runtao Li, Jingrong Cui, Analysis of 4-methyl-piperazine-1-carbodithioic acid 3-cyano-3,3- diphenyl-propyl ester hydrochloride and its major metabolites in rat plasma and tissues by LC-MS/MS,Journal of Separation Science, 2007, 30(13): 2080-2085

23．Xiaomei Jiang,Xiaomei Ling, Fangbin Han, Detao Kong, Xiaoyan Liu, Runtao Li, Jingrong Cui, Studies on the metabolism of 4-methyl-piperazine-1- carbodithioc acid 3-cyano-3,3-diphenylpropyl ester hydrochloride in rat bile by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry,Rapid Communications in Mass Spectrometry. 2007, 21(10): 1599-1605

24．Xiaomei Jiang,Xiaomei Ling,Fangbin Han, Runtao Li, Jingrong Cui, Studies on the metabolism of 4-methyl-piperazine- 1-carbodithioc acid 3-cyano-3,3-diphenylpropyl ester hydrochloride in rats by high-performance liquid chromatography/electrospray ionization tandem mass spectrometry,Journal of Pharmaceutical and Biomedical Analysis, 2007, 44(5): 1127-1132

25．Xiaomei Ling, Yi Liu, Hui Fan, Yingcheng Zhong, Dan Li, Ying Wang, Studies on interactions of programmed cell death 5 (PDCD5) and its related peptides with heparin by capillary zone electrophoresis,Analytical and Bioanalytical Chemistry, 2007, 387(3): 909-916

26．Liu Yi, Shi Haiming, Sun Zhe,Xiaomei Ling, Tu Pengfei, Enantiomer separation of the four diastereomers of guaiacyl glycerol from Hydnocarpus annamensis by capillary electrophoresis with HP-β-CD as chiral selector,Journal of Chromatography Science, 2007, 45(9): 605-609

27．Yi Liu, Haiming Shi, Zhe Sun,Xiaomei Ling, Pengfei Tu, Enantiomer separation of the four stereoisomers of 1-(4-hydroxy-3- methoxy)-phenyl-2- [4-(1,2,3-trihydroxy-propyl)-2-methoxy]-phenoxy-1,3-propandiol from Hydnocarpus annamensis by capillary zone electrophoresis with HP-β-CD as chiral selector,Chromatographia, 2006, 64(7-8): 483-486

28．Yi Liu, Xianyin Lai,Xiaomei Ling, Yuying Zhao, Jingrong Cui, Interactions Between Thrombin with Flavonoids from Abelmoschus manihot (L.) Medicus by CZE,Chromatographia, 2006, 64(1-2): 45-50.

29．Liu Yi, Zhang Yuan , Liu Xiaoming,Xiaomei Ling, Tu Pengfei, Cui Jingrong, Interactions between thrombin and natural proudcts of Coreopsis tinctoria Nuttt. & Cistanche deserticola Ma. in capillary zone electrophoresis,Journal of Chinese Pharmaceutical Sciences, 2006, 15(1): 38-44.

30．王皎, 李晓娜, 张建美,凌笑梅, 李润涛, 崔景荣，RP-HPLC法测定TM208的含量及有关物质，药物分析杂志，2011,31（1）：99-102

31．王斯婷，李晓娜，王皎，凌笑梅, 代谢组学及其分析技术,药物分析杂志，2010，30（9）：1792-1799

32．凌笑梅, 刘一, 赖先银, 张媛, 刘晓明, 屠鹏飞, 赵玉英, 崔景荣, 采用毛细管电泳方法以凝血酶为靶筛选天然药物提取化合物,高等学校化学学报, 2007, 28(2): 234-237.

33．刘一，凌笑梅，崔景荣. 毛细管电泳技术在研究生物分子间相互作用和药物筛选中的应用,药物分析杂志，2006, 26(9): 1350-1357

34．姜小梅，凌笑梅，李润涛，崔景荣. 高效液相色谱－电喷雾离子阱质谱法分析大鼠体内4-甲基哌嗪-1-二硫代甲酸-(3-氰基-3，3-二苯基)丙酯盐酸盐及其主要代谢物，药学学报，2006，41(11): 1084-1089^[1]